polymorphism of insulin-like growth factor binding protein-3 (igfbp-3) gene associated with growth traits in cyprinus carpio

Authors

farzaneh mehrabi laboratory of molecular genetics and animal biotechnology, department of animal sciences, college of animal science and fisheries, sanru, sari, iran.

mohammad kazem khalesi department of fisheries, faculty of animal science and fisheries , sari university of agricultural sciences and natural resources (sanru), sari, iran

ayoub farhadi laboratory of molecular genetics and animal biotechnology, department of animal sciences, college of animal science and fisheries, sanru, sari, iran.

abstract

the genetic polymorphism of insulin-like growth factor binding protein ( igfbp-3 ) and its association with the growth traits namely condition factor, body weight, and total length were investigated in common carp cyprinus carpio using pcr- single strand conformation polymorphism (sscp) technique. caudal fin clips from 120 c. carpio , aging 5 months, were used in this study and a 330 bp dna fragment was amplified from 5'-utr of carp igfbp-3 . sscp genotyping yielded seven different banding patterns namely a, b, c, d, e, f, and g with frequencies of 14.16, 12.5, 21.67, 9.17, 11.67, 10.0, and 20.84, respectively. the obtained results showed a relatively high polymorphic behavior of 5'-utr regulatory region of studied locus; therefore it shows the importance of this region in regulation of transcription of igfbp-3 gene in carp.  marker-trait association showed significant relationships between the detected genotypes and condition factor (cf). igfbp-3 gene polymorphism in joint with cf signifies relatively high genetic diversity and significant potential for marker-assisted selection in this species.

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Journal title:
iranian journal of ichthyology

جلد ۲، شماره ۳، صفحات ۱۷۲-۱۷۶

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